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Literature summary extracted from

  • Redzynia, I.; Ljunggren, A.; Bujacz, A.; Abrahamson, M.; Jaskolski, M.; Bujacz, G.
    Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases (2009), FEBS J., 276, 793-806.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.2 a complex of chagasin, a protein inhibitor from Trypanosoma cruzi, and papain is crystallized by using the hanging-drop vapor diffusion method at 18°C, the high-resolution crystal structure shows an inhibitory wedge comprising three loops, which forms a number of contacts responsible for the high-affinity binding, the present chagasin-papain complex provides a reliable model of chagasin-cruzipain interactions Carica papaya

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 chagasin protease inhibitor, inhibits all C1 family proteases Carica papaya
3.4.22.2 cystatin B
-
 Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.06
-
 carboxybenzoyl-Phe-Arg-7-(4-methyl)coumarylamide 100 mM sodium phosphate buffer, pH 6.0, containing 1 mM dithiothreitol and 1 mM EDTA Carica papaya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.2 additional information Carica papaya papain is the founding member of the large C1 family of papain-like cysteine proteases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya P00784
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.2 active papain is prepared from the commercial papaya latex enzyme through affinity purification on Sepharose 4B Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 commercial preparation papaya latex enzyme Carica papaya
-
3.4.22.2 latex
-
 Carica papaya
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.2 -80°C, purified enzyme can be activated to at least 65% of its original activity after storage at -80°C for up to 3 months Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 carboxybenzoyl-Phe-Arg-7-(4-methyl)coumarylamide + H2O fluorogenic substrate Carica papaya carboxybenzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.2 additional information papain is the founding member of the large C1 family of papain-like cysteine proteases Carica papaya ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.2 papain
-
 Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 6
-
 assay at Carica papaya

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.2 0.000000036
-
 chagasin inactivation of papain is very fast, rate constant for inactivation is 1500 mM/s Carica papaya